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:: Volume 1, Issue 1 (4-2023) ::
injbir 2023, 1(1): 13-21 Back to browse issues page
mtDNA marker reveals the first record of Sepiella japonica (Sasaki, 1929) in the marine waters of Pakistan
A. Sulaman , H.H. Shad , M. Shafi , M.S. Chang , F. Muhammad *
Center of Excellence in Marine Biology, University of Karachi, Karachi-75270, Pakistan
Abstract:   (968 Views)
Cephalopods are the most intelligent, mobile and also the largest of all mollusks, with all members marine. Cuttlefish is a valuable fishery source and is used as human food because of its high nutritive value. Previously, only one Sepiidae species, Sepiella inermis, had been reported in Pakistan. Morphological species identification is now being replaced by molecular-based approaches due to their fast and reliable results. Here, we used mitochondrial fragments, cytochrome c oxidase I (COI) to investigate whether Sepiella japonica could be identified by the DNA barcoding technique. In the present investigation, a 588 bp fragment of Cytochrome oxidase subunit 1 sequence revealed 98.40% identity with S. japonica. The evolutionary distance between the S. japonica from Pakistan and India is 0.012. The neighbor-joining tree showed a close relationship between sequences of this species from India and Pakistan. The S. japonica sequence from this region was submitted to NCBI under accession number ON430600. To the best of our knowledge, this study is the first that report S. japonica in Pakistani coastal waters. Presence of this commercially important species will be a valuable addition to the Pakistani coastal waters. Molecular information provides important assistance for taxonomic decisions. 
 
Keywords: Sepiella japonica, CO1, DNA barcoding, Pakistan, Cuttlefish, PCR
Full-Text [PDF 428 kb]   (284 Downloads)    
Type of Study: Research | Subject: General
Received: 2023/06/11 | Accepted: 2022/07/6 | Published: 2022/07/6
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Sulaman A, Shad H, Shafi M, Chang M, Muhammad F. mtDNA marker reveals the first record of Sepiella japonica (Sasaki, 1929) in the marine waters of Pakistan. injbir 2023; 1 (1) :13-21
URL: http://injbir.com/article-1-24-en.html


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