fa عمومى General پژوهشي Research <span style="font-size:11pt"><span style="line-height:120%"><span style="font-family:Calibri,sans-serif"><span lang="TR" style="font-size:12.0pt"><span style="line-height:120%"><span style="font-family:&quot;Times New Roman&quot;,serif">Acute Hepatopancreatic Necrosis Disease (AHPND) is one of the most severe bacterial diseases affecting global aquaculture of whiteleg shrimp (<i>Litopenaeus vannamei</i>). The disease is primarily associated with specific strains of <i>Vibrio parahaemolyticus</i> carrying the PirA and PirB toxin genes, and can result in mortality rates approaching 100% in affected farms. The increasing concern over antibiotic resistance and environmental impacts of antimicrobial misuse has driven interest in probiotics as a sustainable alternative strategy. Recent studies have indicated that multi-strain probiotics and synbiotics may provide superior protective effects compared with single-strain formulations in controlling AHPND. This review compiles and critically analyzes published research on the efficacy of multi-strain probiotics and synbiotics in reducing AHPND incidence and severity in <i>L. vannamei</i>. The findings suggest that microbial consortia and synbiotic formulations may enhance disease resistance through improved gut microbiota balance, pathogen inhibition, and immune modulation in shrimp. Overall, multi-strain probiotic and synbiotic strategies represent promising sustainable approaches for the management of AHPND in shrimp aquaculture systems.</span></span></span></span></span></span><br> &nbsp; AHPND, Multi-strain probiotics, Synbiotics, Litopenaeus vannamei, Vibrio parahaemolyticus, shrimp aquaculture. 1 14 http://injbir.com/browse.php?a_code=A-10-1-43&slc_lang=fa&sid=1 S. Tamadoni Jahromi 1003194753284600656 1003194753284600656 Yes Persian Gulf and Oman Sea Ecology Research Center, Agricultural Research Education and Extension Organization (AREEO), Bandar Abbas, Iran S. Pourmozaffar 1003194753284600657 1003194753284600657 No Persian Gulf Mollusks Research Station, Persian Gulf and Oman Sea Ecology Research Center, Iranian Fisheries Sciences Research Institute, Agricultural Research Education and Extension Organization (AREEO), Bandar-e-Lengeh, Iran R. Nahavandi 1003194753284600658 1003194753284600658 No Animal Science Research Institute of Iran (ASRI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran S. Nooraei 1003194753284600659 1003194753284600659 No Studet of Immunology, Faculty of Veterinary Medicine, Urmia University, Iran N. Barzkar 1003194753284600660 1003194753284600660 No Borneo Marine Research Institute, Higher Institution Centers of Excellence, University Malaysia Sabah, Kota Kinabalu 88400, Sabah, Malaysia M. Nazemi 1003194753284600661 1003194753284600661 No Persian Gulf and Oman Sea Ecology Research Center, Agricultural Research Education and Extension Organization (AREEO), Bandar Abbas, Iran G.E. Elshopakey 1003194753284600662 1003194753284600662 No Department of Clinical Pathology, Faculty of Veterinary Medicine, Mansoura University, 35516, Egypt S. Elbahnaswy 1003194753284600663 1003194753284600663 No Department of Aquatic Animal Medicine, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt