other عمومى General پژوهشي Research <span style="font-size:11pt"><span style="line-height:120%"><span style="font-family:Calibri,sans-serif"><span lang="TR" style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times="">Based on the 16S rRNA genome of mitochondrial DNA, this study examined the genetic diversity of <i>Penaeus vannamei</i> broodstocks to determine genetic indicators. This study included two populations, High Health (H) and Molokai (M), of the F3 and F5 generations, respectively. Extraction of DNA from samples was performed using a commercial kit. A pair of special primers amplified the 16S rRNA gene. Then, a 16S rRNA fragment of mitochondrial DNA was sequenced. The World Gene Bank has accession numbers LC468129.1 and LC468130.1 for these sequences. Based on the 486 loci identified, there are 484 conserved loci and two variable loci, each of which contains phylogenetic information under parsimony conditions, and 18 singletons. The similarity between LC468129.1 and LC468130.1 with LC121701.1 was 99.49 and 98.99%, respectively. In conclusion, Neighbor-joining algorithms identified each population with accession number LC468129.1 and LC468130.1 with 64% and 66% in separate branches, respectively.</span></span></span></span></span></span> Penaeus vannamei, 16S rRNA, Genetic indicators, Phylogenetic 1 12 http://injbir.com/browse.php?a_code=A-10-1-1&slc_lang=other&sid=1 M.Kh. Pazir 100319475328460046 100319475328460046 Yes Iran Shrimp Research Center, Iranian Fisheries Science Research Institute, Agricultural Research, Education & Extension Organization (AREEO), Bushehr, Iran. A. Dashtiannasab 100319475328460047 100319475328460047 No Iran Shrimp Research Center, Iranian Fisheries Science Research Institute, Agricultural Research, Education & Extension Organization (AREEO), Bushehr, Iran. S. Kakoolaki 100319475328460048 100319475328460048 No Iranian Fisheries Science Research Institute, Agricultural Research, Education & Extension Organization (AREEO), Tehran, Iran. E. Mohammadi 100319475328460049 100319475328460049 No Iran Shrimp Research Center, Iranian Fisheries Science Research Institute, Agricultural Research, Education & Extension Organization (AREEO), Bushehr, Iran. S. Pourmozaffar 100319475328460050 100319475328460050 No Persian Gulf Mollusks Research Station, Persian Gulf and Oman Sea Ecology Research Center, Iranian Fisheries Sciences Research Institute, Agricultural Research Education and Extension Organization (AREEO), Bandar-e-Lengeh, Iran. I. Hakiminia 100319475328460051 100319475328460051 No University of Applied Sciences & Technology of Tangestan Pasargad branch, University of Applied Sciences & Technology of Bushehr. A. Hemati 100319475328460052 100319475328460052 No Iranian Fisheries Science Research Institute, Agricultural Research, Education & Extension Organization (AREEO), Tehran, Iran.