en تخصصي Special پژوهشي Research <span style="font-size:12pt"><span style="font-family:&quot;Times New Roman&quot;,serif"><span style="color:black">The <em>FAE1</em> gene encodes a 3-ketoacyl-CoA synthase involved in the elongation of fatty acid chains and the biosynthesis of very-long-chain fatty acids (VLCFAs), including erucic acid. Modifying <em>FAE1</em> through genome-editing technologies offers a promising strategy for tailoring seed oil composition in oilseed crops for specific nutritional and industrial applications. This review examines recent advances in CRISPR/Cas-mediated editing of <em>FAE1</em> and its effects on seed fatty acid profiles, with particular emphasis on <em>Camelina sativa</em>, <em>Brassica napus</em> and <em>Brassica juncea</em>. Relevant studies were identified through searches of major scientific databases, including PubMed, Scopus, Web of Science and Google Scholar. Published research on CRISPR/Cas-mediated editing of <em>FAE1</em> in oilseed crops, together with relevant molecular and functional studies using <em>Arabidopsis thaliana</em> as a model plant, was critically evaluated. Evidence from <em>C. sativa</em> demonstrates that simultaneous disruption of its three <em>FAE1</em> homeologs can reduce seed VLCFA content to less than 2% of total fatty acids, compared with more than 22% in wild-type plants, accompanied by increased proportions of C18 unsaturated fatty acids. Similarly, targeted disruption of <em>FAE1</em> homologs in <em>B. napus</em> and <em>B. juncea</em> has resulted in substantial reductions in seed erucic acid, with some edited lines exhibiting nearly complete elimination of this fatty acid and increased oleic acid content. However, the magnitude of these changes varies among species, cultivars and editing strategies. This review also discusses the implications of <em>FAE1</em> modification for nutritional oil quality, industrial applications, seed oil content and agronomic performance. Despite promising advances, further research is required to optimize editing efficiency, improve transformation and regeneration protocols, assess potential off-target effects, establish the stability and inheritance of edited traits, and evaluate their effects on seed composition and crop performance. Overall, CRISPR/Cas-mediated editing of <em>FAE1</em> represents a valuable approach for the targeted improvement of oilseed crops and the development of vegetable oils with tailored fatty acid profiles.</span></span></span> FAE1, CRISPR/Cas9, genome editing, oilseed crops, very-long-chain fatty acids, erucic acid, fatty acid composition. 0 0 http://injbir.com/browse.php?a_code=A-10-66-1&slc_lang=en&sid=1 Reyhaneh Yarmohammadi Jalali ryhanhyarmhmdy@gmail.com Yes Master of Biochemistry, Faculty of Basic Sciences, Arak University,Arak,Iran F. Jalalimanesh No PhD student of Cellular and Molecular Biology, Graduate University of Advanced Technology, Kerman, Iran