International Journal of Biology Reports

en Anesthetic effect of ethanol, MS222 and cold maintenance on freshwater crayfish (Astacus leptodactylus) عمومى General پژوهشي Research Freshwater crayfish from the Aras Dam are considered one of the country's high-value fishery export resources. Freshwater crayfish are exported live and at low temperatures. Limited studies have been conducted on their welfare and the use of anesthetics in stressful situations such as handling, cooking, manipulation, sampling, and transportation. The present study was conducted to determine the effective dose and time of anesthesia induction and recovery time using different levels of MS-222 (levels 0, 50, 100, 200, 400, 800 and 1200 mg/L), ethanol (levels 0, 50, 100, 200, 400, 800, 1200 and 1600 mg/L) and cold (temperatures 4, 6, 8 and 25°C as control) in freshwater crayfish (Astacus leptodactylus). In this study, the duration of anesthesia induction and recovery time of freshwater crayfish were investigated with the aim of determining the appropriate anesthetic concentration for young crayfish weighing approximately 20±3 grams. The results of the present study showed that the use of high levels of MS-222 was not effective in anesthetizing freshwater crayfish. The use of ethanol at high levels (30%) was also able to anesthetize crayfish in a short time, but at low levels this process required a long time. The results also showed that a low temperature of 4°C had the shortest induction time and the fastest recovery from anesthesia compared to MS-222 and ethanol. Therefore, the use of ethanol due to high stress induction and MS-222 due to high price and low performance in freshwater crayfish anesthesia is not recommended. Anesthesia, MS-222, Ethanol, Cold, Freshwater crayfish 63 72 http://injbir.com/browse.php?a_code=A-10-48-1&slc_lang=en&sid=1 M. Seidgar seidgar2011@gmail.com 1003194753284600608 1003194753284600608 Yes National Artemia Research Center, Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization, Urmia, Iran. R. Jalili re.jalili@gmail.com 1003194753284600609 1003194753284600609 No National Artemia Research Center, Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization, Urmia, Iran. A. Nekoueifard dr.nekuiefard@gmail.com 1003194753284600610 1003194753284600610 No National Artemia Research Center, Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization, Urmia, Iran. S. Mirzargar zargarm@ut.ac.ir 1003194753284600611 1003194753284600611 No Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. A. Abbaspour Anbi asadanbi1208@gmail.com 1003194753284600612 1003194753284600612 No National Artemia Research Center, Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization, Urmia, Iran. Zh. Alizadeh Osalou alizadeh.zhaleh@yahoo.com 1003194753284600613 1003194753284600613 No National Artemia Research Center, Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization, Urmia, Iran.