en تخصصي Special كاربردي Applicable <span style="font-size:11pt"><span style="line-height:120%"><span style="font-family:Calibri,sans-serif"><span lang="TR" style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Acute Hepatopancreatic Necrosis Disease (AHPND) is a severe bacterial infection that threatens shrimp aquaculture, causing significant economic losses globally. The disease is attributed to specific strains of <i>Vibrio</i> spp. carrying the <i>pVA1</i> plasmid, which encodes <i>PirAB</i> toxins responsible for hepatopancreatic necrosis in shrimp. Early detection of AHPND is crucial for effective disease management, yet several challenges hinder accurate and timely diagnosis. This article examines key diagnostic challenges, including genetic variability in AHPND-causing bacteria, inconsistencies in toxin production, environmental influences, and limitations of current molecular detection methods. Additionally, alternative diagnostic approaches such as LAMP, CRISPR/Cas12a assays, and immunoassays are discussed for their potential to improve detection accuracy. Future research should focus on developing more robust and rapid diagnostic tools to overcome the limitations of current methods. Enhanced detection strategies, combined with biosecurity and preventive measures, will be essential in mitigating the impact of AHPND on global shrimp farming.</span></span></span></span></span></span></span> AHPND, Vibrio, PirAB, Diagnosis, Aquaculture. 13 27 http://injbir.com/browse.php?a_code=A-10-25-2&slc_lang=en&sid=1 M.Kh. Pazir dr.pazir@gmail.com 1003194753284600507 1003194753284600507 Yes Shrimp Research Center, Iranian Fisheries Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Bushehr, 75169-89177, Iran A.H. Ahmadi a_ahmadi1985@yahoo.com 1003194753284600508 1003194753284600508 No Department of Biological Science and Technology, Faculty of Nano and Bio Science and Technology, Persian Gulf University, Bushehr, 75169, Iran M.A. Nazari ma.nazari89@gmail.com 1003194753284600509 1003194753284600509 No Shrimp Research Center, Iranian Fisheries Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Bushehr, 75169-89177, Iran E. Mohammadi Em.mohammadi@yahoo.com 1003194753284600510 1003194753284600510 No Shrimp Research Center, Iranian Fisheries Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Bushehr, 75169-89177, Iran M. Mohseni mohsenimail14@gmail.com 1003194753284600511 1003194753284600511 No Shrimp Research Center, Iranian Fisheries Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Bushehr, 75169-89177, Iran Kh. Aeinjamshid khosrow.aein@gmail.com 1003194753284600512 1003194753284600512 No Shrimp Research Center, Iranian Fisheries Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Bushehr, 75169-89177, Iran A. Dashtian Nasab adashtiannasab@gmail.com 1003194753284600513 1003194753284600513 No Shrimp Research Center, Iranian Fisheries Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Bushehr, 75169-89177, Iran O. Jafari jaafari.omid@yahoo.com 1003194753284600514 1003194753284600514 No International Sturgeon Research Institute, Iranian Fisheries Science Research Institute, Agricultural Research (AREEO), Education and Extension Organization, Rasht, Iran S. Pourmozaffar sajjad5550@gmail.com 1003194753284600515 1003194753284600515 No Persian Gulf Mollusks Research Station, Persian Gulf and Oman Sea Ecology Research Center, Iranian Fisheries Sciences Research Institute, Agricultural Research Education and Extension Organization (AREEO), Bandar-e- Lengeh, 75145, Iran